RT-PCR test: NEERI develops simple, fast method of swab collection and processing | India News – Times of India

New Delhi: The National Environmental Engineering Research Institute (NEERI) has developed a simple and fast method of swab collection and processing for RT-PCR coronavirus testing that can be used in rural and tribal areas.
The Council of Scientific and Industrial Research (CSIR) stated that the method is simple, fast, cost-effective, patient-friendly and comfortable. NEERI is a constituent laboratory of CSIR based in Nagpur.
CSIR said that it is suitable for rural and tribal areas, given the minimum infrastructure requirements.
Krishna Khairnar, senior scientist at the Environmental Virology Cell at NEERI, said that the swab collection method requires time. Also, since it is an invasive technique, it is a bit uncomfortable for patients.
“Sometimes, it is also lost in transporting the sample to the collection center. On the other hand, the Céline Gargle RT-PCR method is immediate, comfortable, and patient-friendly. Sampling is done immediately and results will be generated within three. Hours, “he said.
The method is non-invasive and so simple that a patient can collect the sample themselves, Khairnar said.
Collection methods such as nasopharyngeal and oropharyngeal swab collections require technical expertise and are also time-consuming. In contrast, the saline Gargle RT-PCR method uses a simple collection tube filled with saline solution, he noted.
The patient rubs the solution and rinses it inside the tube. This sample in the collection tube is transported to the laboratory where it is kept in a special buffer solution prepared by Neeri at room temperature.
On heating this solution, an RNA template is prepared, which is further processed for reverse transcription-polymerase chain reaction (RT-PCR).
This special method of sample collecting and processing enables the otherwise costly structural requirement of RNA extraction to be saved. Khairnar said the method is also environmentally friendly, as waste production is minimal.
CSIR said that Nagpur Municipal Corporation has given permission to proceed with the law, after which the trial in Neeri has started.

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