IISc study shows forms of SARS-CoV2 S-protein | India News – Times of India

Bengaluru: A research team at the Indian Institute of Science (IISc) has made a significant breakthrough by successfully visualizing various forms or variants of SARS-CoV2’s S-protein – the virus that causes Kovid-19 – which is primarily Exists in two analogies. : Open and closed.
According to IISc, in the study, researchers detected several different intermediate conformations between fully open and closed forms. Furthermore, they show that the different states of both conformations have different binding similarities towards neutralizing antibodies. “The structural insights gained from this study may aid in developing therapeutics,” the IISc stated.
The work, led by Assistant Professor Somnath Dutta at the MIS’s Molecular Biophysics Unit (MBU), has been published in the peer-review scientific journal Structure.
A key feature of SARS-CoV-2 is the presence of spike (S) proteins on the membrane surface that cover the genetic material of the virus. They appear as distinctive crown-like spikes on the viral surface.
And, the S-protein is important for several reasons: it mediates the entry of the virus into the host cell and is also the site where the antibody produced by the host cells is bound to inactivate the virus to neutralize it. Therefore it is important to understand the detailed structure of this protein, the researchers argued.
“Most previous studies on the structure of S proteins have been done on either potential hydrogen 8.0 ((pH 8.0) or pH 4.0 to pH 5.0. But under physiologically relevant conditions the structure of the S-protein – on which the virus infects the host. Cells – Less understood. Recently, Dutta’s team successfully visualized various forms of proteins near physiological pH 7.4 and physiological pH (pH 6.5 and pH 8.0) using a technique called single-particle cryo-electron Has achieved a significant success. Microscopy, “according to IISc.
Pointing out that the said protein is mainly present in two forms, “open and closed”, the institute said that the open conformation is the form that helps bind the protein to the host cell receptor, thus in the cell of the virus. Makes way to enter.
“The team observed that about 68% of the S-proteins are present in the open structure at physiological pH 7.4, but their ratio decreases when the pH is slightly higher (pH 8.0) or lower (pH 6.5). This suggests that The interaction between the S protein and the receptor is more favorable at physiological pH (pH 7.4) than on either side of the biological pH scale, ”the IISc stated.
Other authors of the study are: Ishika Pramanik, Nayanika Sengupta, Suman Mishra, Suman Pandey, Nidhi Girish and Alkata Das.

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